Journal of Food, Agriculture and Environment

Identification and Distribution of ALS resistant Lolium rigidum populations in Saudi Arabia

Author(s):

Abdullah A. Al-Doss, Abdelhalim I. Ghazy *, Abd El-azeem K. Salem, Hussein M. Migdadi, Sulieman A. Al-Faifi

Recieved Date: 2013-07-06, Accepted Date: 2013-10-20

Abstract:

Wild annual ryegrass (Lolium rigidum Gaud) is one of the most common weed species invading cereal crops in Saudi Arabia. During the last decade, several resistances to specific grass weed herbicides appeared within annual ryegrass populations growing in wheat fields. The present work aimed to identify and study the distribution of mutants conferring resistance to ALS herbicides in wild ryegrass populations invading wheat fields in Saudi Arabia using control tests and molecular markers. Seeds of L. rigidum from seven regions in Saudi Arabia and a susceptible accession were used in screening for ALS herbicide resistance. The populations were subjected to two doses (100 and 200 mg a.i/ha) of the ALS herbicide Atlantis™ during three leaf stage in a greenhouse test. The ryegrass populations were also subjected to molecular screening using specific primers published for mutation detection of resistant and tolerant to ALS herbicides. Results of the herbicide experiment showed significant differences among collected populations in response to the herbicide treatment.The most resistant populations were Tabouk and Qassim populations (33% and 30% of survival, respectively). On the other hand, the sensitive population and Harad were very susceptible to herbicide tratment (0% survival). Molecular screening revealed polymorphism among populations for ALS resistant mutants indicating the presence of resistant ryegrass populations in Saudi Arabia. Moreover, ALS mutants were identified in two populations (Tabouk and Qassim) which confirmed the result obtained in the herbicide resistance test. Three out of four mutants were revealed in Aljouf , Hail and Dwadmi. However, Harad and Wadi Aldawaser showed some resistance in two out of four mutations. Mutants conferring resistance to ALS herbicides in wild ryegrass populations invading wheat fields in Saudi Arabia were identified in two out of seven populations (Tabouk and Qassim) at molecular level. This result will help to implement new strategies to manage this weed problem.

Keywords:

Lolium rigidum, acetolactate synthase (ALS) resistance, weed treatments, winter cereals

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Agriculture
Pages: 1311-1314

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