Journal of Food, Agriculture and Environment

The in vitro rooting performance of pear rootstock ‘OHxF 333’ in different rooting procedures

Author(s):

Umran Erturk

Recieved Date: 2013-06-12, Accepted Date: 2013-10-10

Abstract:

The in vitro rooting ability of ‘OHxF 333 pear rootstock’ (Pyrus communis L.) was investigated using indolebutyric acid (IBA) (0, 0.5 and 1.0 mgL^-1), putrescine (160 mgL^-1) and 4 different rooting procedures: the shoots taken from a multiplication medium (A) were transferred to an IBA- and putrescine-containing medium for 35 days; (B) were placed on an IBA- and putrescine-containing medium for 10 days, after which the shoots were transferred to an auxin- and putrescine-free medium for 25 days; (C) were placed on a hormone-free medium for 15 days, after which the shoots were transferred to an IBA- and putrescine-containing medium for 35 days; and (D) were placed on a hormone-free medium for 15 days, after which the shoots were transferred to an IBA- and putrescine-containing medium for 10 days and then transferred to a hormone-free medium for 25 days. Among the various rooting procedures, procedure A gave the highest rooting percentage (49.50%), number of roots (4.09) and length of roots (2.26 cm). The best results for rooting rate (86.86%) and number of roots (10.36) were obtained from the interaction between procedure A and the addition of 1.0 mgL^-1 IBA. The elongation phase used in procedures C and D did not have a superior effect on the rooting rate. Generally, putrescine alone produced a lower rooting rate, number of roots and root length than IBA in all rooting procedures.

Keywords:

In vitro, pear rootstock, rooting, IBA, putrescine

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Agriculture
Pages: 1424-1427

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