Journal of Food, Agriculture and Environment
Assessment of some morphometric changes in pikeperch (Sander lucioperca) spermatozoa, due to dilution and UV-irradiation of the milt
Author(s):
Adrian Grozea *,
Ada Cean, Violeta-Nicoleta Igna, Ioan Banatean-Dunea, Ludovic-Toma CziszterRecieved Date: 2013-06-10, Accepted Date: 2013-09-10
Abstract:The role of some environmental and technological factors on reproductive performances and semen characteristics of pikeperch have been demonstrated by different authors. A good knowledge of the phenomena that occur at spermatozoa level during milt preparation is essential for success of gynogenesis, but there is no information in the literature about the influence of diluents and UV-irradiation on spermatozoa morphometric parameters in this species. The aim of this study was to evaluate the main morphometric parameters of pikeperch spermatozoa, and their dynamics over 17 min after milt collection, dilution and UV-irradiation, these being phases followed by our team in gynogenesis protocol for DNA inactivation in pikeperch spermatozoa. Spermac staining method proved to be the most suitable method for morphometric study on pikeperch sperm allowing morphometric measurements. With time there were some changes in studied morphometric parameters, particularly those related to length and width of sperm head. Milt dilution and its irradiation with UV rays led to an increase of the spermatozoa head length and width. Starting from a mean value of 2.25 ± 0.05 µm in rough milt, the spermatozoa head length reached up 3.75 ± 0.12 µm after milt dilution and UV-irradiation. The head width showed the same trend, starting from 2.11 ± 0.04 µm in rough milt, it reached up 3.49 ± 0.11 µm after milt dilution and UV-irradiation. Milt preparation variants did not significantly influenced sperm tail length, the average value of this parameter varying between 32.52 ± 0.61 µm and 33.93 ± 0.54 µm. The results emphasize that pikeperch sperm preparation in gynogenesis protocol lead to major changes at spermatozoa head level, especially increasing of their head length and width due to dilution and UV-irradiation.
Keywords:Pikeperch,
milt, dilution, UV-irradiation, spermatozoa, morphometric parametersJournal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Environment
Pages: 1853-1857
Full text for Subscribers
Information:
Note to users
The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.
Article purchasing
If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.
Purchase this Article: 20 Purchase PDF Order Reprints for 15
Share this article :