Journal of Food, Agriculture and Environment

Isolation and identification of autotoxic chemicals from Angelica sinensis (Oliv.) Diels

Author(s):

Hui Zhu ^{1, 2}, Shuangtao Wu ², Qinghan Wu ², Changlian Peng ¹*

Recieved Date: 2013-07-10, Accepted Date: 2013-10-10

Abstract:

To uncover the autotoxic mechanism of the continuous cropping obstacle in planting of Angelica sinensis (Oliv.) Diels, the type and structure of autotoxic chemicals from A. sinensis were isolated and identified by column chromatography and tracking bioassay. Using the aboveground parts (stems + leaves) of A. sinensis in vegetative growth stage as donor materials in this experiment, which were extracted with the ratio of material to water is 1:20 under Ultrasonic-assisted conditions, and the water-extraction solution with mass concentration of 0.1 g DW mL^-1 was got after the extracting solution was treated through vacuum filtration twice and pressure-reducing condensation. Petroleum ether phase, ethyl acetate phase and n-butyl alcohol phase were obtained by extracting and isolating with several organic solvents in turn, the ethyl acetate phase was identified as the phase of the strongest autotoxic activity (M = -0.2476) using tracking bioassay, which was isolated by column chromatography method and a total of 8 liquid-fractions were obtained from the ethyl acetate phase, named A, B, C, D, E, F, G and H, respectively. In which liquid-fraction D was proved to be the strongest autotoxic activity fraction (M = -0.6383) and continued to be isolated by column chromatography, the compound D₁ and mixture D_1+X contained D₁ were obtained finally. The structure of D₁ was identified as 4,6-dimethylbicyclo[3.1.0]hex-2-ene-2,6-dimethanol by NMR and MS, which belongs to the monoterpenoids. It is possible that there is synergistic autotoxic effect between the compound D₁ and other chemicals from A. sinensis according to the bioassay results.

Keywords:

Angelica sinensis (Oliv.) Diels, Chinese herbal medicine, autotoxicity, continuous cropping obstacle, column chromatography, bioassay

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Environment
Pages: 2136-2140

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