Journal of Food, Agriculture and Environment

Vol 12, Issue 2,2014
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Evaluation of the potential genotoxicity of antibiotics alternative probiotics used in livestock and poultry


Mohammed Hamed Zeiny Mutwakil 1, Jamal Sabir Mohamed Sabir 1, Saleha Yahya Mohamed Al Akilli 1, Aida Ibrahim El Makawy 2, Mohamed Morsi Mohamed Ahmed 1, 3*

Recieved Date: 2014-01-08, Accepted Date: 2014-04-11


Probiotics are used in poultry industry to overcome a ban for the use of antibiotic for disease prevention and growth enhancing supplements. Biomin Imbo and Primalac are used as dietary commercial non-antibiotic growth promoter for poultry. The objective of this study was to evaluate the potential genotoxicity of Biomin-Imbo and Primalac in mice. Male mice were feed basal diet supplemented with 1.5 and 3 g/kg of both Biomin Imbo and Primalac for 6 weeks. Positive control animals were feed on basal diet and administered antibiotic neomycin in water. The study was conducted to assess DNA damage by micronucleus test in bone marrow cells, DNA fragmentation by diphenylamine (DPA) and DNA fingerprinting using random amplified polymorphic DNA (RAPD-PCR) analysis in liver cells. Results revealed that Biomin Imbo and Primalac induced dose dependent increase in the frequencies of micro nucleated polychromatic erythrocytes (MNPCEs), DNA fragmentation percentage and appearance of some changes in polymorphism band patterns including deletion of stable band or insertion of new bands. Noteworthy that the increment in DNA damage induced by both Biomin Imbo or Primalac did not reach to those induced by neomycin. We concluded that the high dose repeated exposure of Biomin Imbo and Primalac may have the potential to induce cytotoxic and moderate genotoxic effects in bone marrow cells of male mice.


Antibiotics alternatives, commercial probiotics supplements, poultry, DNA, fragmentation-MNPCEs-RAPD

Journal: Journal of Food, Agriculture and Environment
Year: 2014
Volume: 12
Issue: 2
Category: Agriculture
Pages: 591-598

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