Journal of Food, Agriculture and Environment

Molecular detection of Pasteuria penetrans isolates parasitizing Meloidogyne incognita in Al-Qassim area, Saudi Arabia

Author(s):

Suloiman Al-Rehiayani ^1,2*, M. I. Motawei ^1,2

Recieved Date: 2018-01-10, Accepted Date: 2018-03-27

Abstract:

The root-knot nematodes, Meloidogyne spp. are serious parasites  in a variety of important agricultural crops in Saudi Arabia and around the world. Pasteuria penetrans is an endospore-forming bacterial parasite of Meloidogyne spp. This organism is among the most promising agents for the biological control of root-knot nematodes. Meloidogyne incognita populations with Pasteuria attached to their cuticles were collected from several locations in Al-Qassim area, Saudi Arabia. To amplify DNA from five isolates of P. penetrans infecting  M. incognita in Al-Qassim area, Universal primers for the 16S rDNA gene were used. The results indicated that the two primers (39F/1492R and 27F/1388R) were able to detect  the five isolates of Pasteuria from M. incognita in Al-Qassim area. A nucleotide sequence was obtained for the gene encoding the 16S rDNA from each isolate. The sequences of 16S rDNA of five isolates showed the presence of P. penetrans. Comparisons between isolates were investigated using ITS-RFLP. Polymorphic ITS-RFLP patters from the four restriction endonucleases allowed differentiation of the isolates of P. penetrans. Based on cluster analysis of the obtained fragments of ITS-RFLP, the isolates of P. penetrans fall into two distinct clusters. Because of the high specificity and sensitivity of ITS-RFLP, it was possible to discriminate between P. penetrans isolates.

Keywords:

Biological control, Meloidogyne incognita, molecular biology,  Pasteuria penetrans

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Journal: Journal of Food, Agriculture and Environment
Year: 2018
Volume: 16
Issue: 2
Category: Agriculture
Pages: 119-123

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