Journal of Food, Agriculture and Environment

Vol 5, Issue 1,2007
Online ISSN: 1459-0263
Print ISSN: 1459-0255

Incidence of verocytotoxin (Shiga-like toxin) producing Escherichia coli 0157:H7 isolated from diarrhoeal patients and its health implication


J. E. Ngbede 1*, I. A. Jideani 2, E. B. Agbo 2

Recieved Date: 2006-09-02, Accepted Date: 2006-11-29


Diarrhoeal disease in developing countries is caused by an increasing long list of bacteria, viral and parasitic enteropathogens. Escherichia coli is a type of bacteria present in all animal gut, but some strains cause diarrhoeal disease. There has been considerable progress during recent years regarding the role of toxins in diarrhoeal diseases. Enterohaemorrhagic E. coli (EHEC) is known to produce verocytotoxin (VT) which inhibits protein synthesis and cause endothelial damage of small blood vessels leading to local intravascular coagulation and fibrin deposition in central nervous system (CNS), gut and kidney resulting in severe illness. The aim of this study was to determine the incidence of verocytotoxin produced by E. coli 0157:H7 isolated from diarrhoeal patients and its health implication. During the study period, 850 diarrhoeal specimens were analysed using sorbitol MacConkey agar, latex agglutination test and antiserum-sorbitol fermentation medium. Of these specimens 26 (3.1%) were found to be infected with E. coli 0157:H7 among other enteropathogens. The E. coli 0157:H7 isolates were analysed for presence of verocytotoxin using standard VTEC-RPLA assay. VT-1 was detected from 3/26 (11.5%), VT-2 from 17/26 (65.4%) and VT-1 and VT-2 from 6/26 (23.1%) of isolates. We recommend that hospital laboratories should consider performing stool culture for verocytoxoxin producing E. coli (VTEC), especially serotype 0157:H7, either routinely or on selected patients to avoid the severe consequences of toxins produced by this strain of E. coli.


Enterohaemorrhagic E. coli 0157:H7, verocytotoxin, health implication

Journal: Journal of Food, Agriculture and Environment
Year: 2007
Volume: 5
Issue: 1
Category: Food and Health
Pages: 49-51

Full text for Subscribers

Note to users

The requested document is freely available only to subscribers/registered users with an online subscription to the Journal of Food, Agriculture & Environment. If you have set up a personal subscription to this title please enter your user name and password. All abstracts are available for free.

Article purchasing

If you like to purchase this specific document such as article, review or this journal issue, contact us. Specify the title of the article or review, issue, number, volume and date of the publication. Software and compilation, Science & Technology, all rights reserved. Your use of this website details or service is governed by terms of use. Authors are invited to check from time to time news or information.

Purchase this Article:   20 Purchase PDF Order Reprints for 15

Share this article :