Journal of Food, Agriculture and Environment

Simultaneous analysis of glycinebetaine and trigonelline in Eucalyptus citriodora Hook (F.) in response to drought stress using reversed-phase high-performance liquid chromatography

Author(s):

Maher J. Tadros ¹, Barakat M. Shabsoug ²*

Recieved Date: 2013-07-22, Accepted Date: 2013-10-12

Abstract:

Glycinebetaine and trigonelline are quaternary ammonium compounds (QACs), which serve as osmoprotectants for plants grown under various environmental stress conditions. We have developed a simple and rapid isocratic high-performance liquid chromatography (HPLC) method for the simultaneous analysis of these compounds. The method utilizes a reversed-phase C18 column, a mobile phase consisting of 1-heptanesulfonic acid/ sodium sulfate, and UV detection. We have investigated the effect of column length, pH of the mobile phase, and use of an organic modifier on the elution profile of trigonelline. The effect of detection wavelength on the sensitivity of glycinebetaine quantification has also been explored. The standard conditions with 10 mM 1-heptanesulfonic acid (sodium salt)/5 mM sodium sulfate (pH 3.7) at a flow-rate of 0.8 mL min^-1 and detection at 200 nm provided good separation and response factors for the two QACs. Under these conditions, the detection limit is 1 and 77 µM for trigonelline and glycinebetaine, respectively. In addition to method development and calibration on known standards of the compounds, we have used this HPLC method to detect QACs in extracts of plants grown under greenhouse conditions successfully.

Keywords:

Glycinebetaine, eucalyptus, desert species, drought stress, water stress, HPLC

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Journal: Journal of Food, Agriculture and Environment
Year: 2013
Volume: 11
Issue: 3&4
Category: Environment
Pages: 2551-2555

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